Author/Authors :
Dadashpour، A نويسنده Young Researchers Club, Shahre-Qods Branch, Islamic Azad University, Tehran, Iran. , , MohammadAmooie، H نويسنده Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. , , Jouki، M نويسنده Young Researchers Club, Shahre-Qods Branch, Islamic Azad University, Tehran, Iran. , , Khazaei، N. نويسنده Young Researchers Club- Islamic Azad University- Shahr-e-qods Branch, Tehran, I. R. Iran, PO box 37515-374, Tehran, Iran. ,
Abstract :
The anthocyanins of the fruit skin of Iranian forest pear (Sange Khouj)
were separated, identified and quantified using high-performance liquid chromatography
/ diode array detection (HPLC / DAD) and HPLC / electrospray
ionization / mass spectrometry (HPLC / ESI / MS). The individual anthocyanins
were identified by comparing their mass spectral data and retention times with
those of standards and published data. Cyanidin-3-galactoside was the major
compound, taking up 93% of the total anthocyanin content. The content of
Cyanidin-3-galactoside reached 23.7 ± 3.2 mg 100 g-1 fresh weight, followed
by the eighth peak (2.14%) and Cyanidin-3-glucoside (1.23%). Pelargonidin-
3-rutinoside was identified for the first time in ordinary pear (P. communis L).
DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant
power) assays showed that the extract from the fruit peel of ‘Sange Khouj’
contained anthocyanins and possessed high antioxidant capacity.
