Title of article
The Role of Adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro
Author/Authors
Safitri ، E. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Utama ، S. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Bumi ، C. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Mulyani ، S. W. M. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Susilowati ، H. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Retnowati ، E. نويسنده Department of Clinical Pathology, Faculty of Medicine, Airlangga University. , , Purwati.، - نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. , , Prasetyo ، R. H. نويسنده Departement of Parasitology School of Medicine, Airlangga University. , , Hariadi ، M. نويسنده Department of Veterinary Reproduction Faculty of Veterinary Med, Airlangga University. , , Rantam ، F. A. نويسنده Stem Cells Research Divison of Institude Tropical Disease (ITD), Airlangga University. ,
Issue Information
روزنامه با شماره پیاپی 0 سال 2013
Pages
5
From page
318
To page
322
Abstract
The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).
Journal title
Journal of Animal Production Advances (JAPA)
Serial Year
2013
Journal title
Journal of Animal Production Advances (JAPA)
Record number
1017655
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