Molecular Genetic Analysis Based on Java Green Peacock (Pavo Muticus) Mitochondrial D-Loop Efforts as a Basis for Domestication in Probolinggo, East Java Indonesia

Besides hunting, habitate’s degradation and extinction were the main important factors lead to declination of green peacocks population. Distance which separate populations in small island also become contact barrier amongs the rest populations. These cause mating between close relatives which will lead to genetic downgrade and local extinction. This study aimed to analyze whether peacocks from breeding cause changing in genetic differences. Six green peacocks breeded from Probolinggo, East Java, Indonesia were being used in this study. Its feather (calamus) were extracted for further examination. Polymerase Chain Reaction (PCR) technique with D-loop mitochondrial-based primer was used to investigate the genetic differences of species amongs green peacock. PCR products (330 bp) were analyzed using gel electrophoresis 1%, it were then purified and labeled for the data sequencing. Sequencing data obtained reflects base order, which will be used to analyze genetic difference amongs species (phylogenetic analysis) with the software program of Genetix Mac Ver 10.0. A green peacock (Afropavo congensis) with the code of DQ 834 507 was being used as a reference. The results showed that the feathers from the six green peacocks breeded in Probolinggo, East Java, Indonesia were genetically different with green peacock which has been reported to GenBank with the code of DQ 834 507. Futhermore, although amongs the six samples which were predicted to have close genetic relationship, but the code of MJM-1; MJMJ-1; MJA-1; MJMA-1 were considered as young clusters / F2, while code MJD2 and MJD-1-1 were considered as adult clusters / F1. Breeding can cause changing in genetic difference, which was showed in this study that peacocks breeded in Probolinggo, East Java, Indonesia were genetically different compare to reference DQ 834 507.