Author/Authors :
Jeffrey R. Holt، نويسنده , , Susan K.H. Gillespie، نويسنده , , D.William Provance Jr.، نويسنده , , Kavita Shah، نويسنده , , Kevan M. Shokat، نويسنده , , David P. Corey and Klaus Schulten، نويسنده , , John A. Mercer، نويسنده , , Peter G. Gillespie، نويسنده ,
Abstract :
Myosin-1c (also known as myosin-Iβ) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N6-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.
