Author/Authors :
Wen-Tao Peng، نويسنده , , Mark D. Robinson، نويسنده , , Sanie Mnaimneh، نويسنده , , Nevan J. Krogan، نويسنده , , Gerard Cagney، نويسنده , , Quaid Morris، نويسنده , , Armaity P. Davierwala، نويسنده , , J?rg Grigull، نويسنده , , Xueqi Yang، نويسنده , , Wen Zhang، نويسنده , , Nicholas Mitsakakis، نويسنده , , Owen W. Ryan، نويسنده , , Nira Datta، نويسنده , , Vladimir Jojic، نويسنده , , Chris Pal، نويسنده , , Veronica Canadien، نويسنده , , Dawn Richards، نويسنده , , Bryan Beattie، نويسنده , , Lani F. Wu، نويسنده , , Steven J. Altschuler، نويسنده , , et al، نويسنده ,
Abstract :
Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA.
