Author/Authors :
Justin Lamb، نويسنده , , Sridhar Ramaswamy، نويسنده , , Heide L. Ford، نويسنده , , Bernardo Contreras، نويسنده , , Robert V. Martinez، نويسنده , , Frances S. Kittrell، نويسنده , , Cynthia A. Zahnow، نويسنده , , Nick Patterson، نويسنده , , Todd R. Golub، نويسنده , , Mark E. Ewen، نويسنده ,
Abstract :
Here we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPβ/Nf-Il6, participates in the consequences of cyclin D1 overexpression. Functional analyses confirmed the involvement of C/EBPβ in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.
