Author/Authors :
Saskia Houwing، نويسنده , , Leonie M. Kamminga، نويسنده , , Eugene Berezikov، نويسنده , , Daniela Cronembold، نويسنده , , Angélique Girard، نويسنده , , Hans van den Elst، نويسنده , , Dmitri V. Filippov، نويسنده , , Heiko Blaser، نويسنده , , Erez Raz، نويسنده , , Cecilia B. Moens، نويسنده , , Ronald H.A. Plasterk، نويسنده , , Gregory J. Hannon، نويسنده , , Bruce W. Draper، نويسنده , , René F. Ketting، نويسنده ,
Abstract :
Piwi proteins specify an animal-specific subclass of the Argonaute family that, in vertebrates, is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is expressed in both the male and the female gonad and is a component of a germline-specifying structure called nuage. Loss of Ziwi function results in a progressive loss of germ cells due to apoptosis during larval development. In animals that have reduced Ziwi function, germ cells are maintained but display abnormal levels of apoptosis in adults. In mammals, Piwi proteins associate with approximately 29-nucleotide-long, testis-specific RNA molecules called piRNAs. Here we show that zebrafish piRNAs are present in both ovary and testis. Many of these are derived from transposons, implicating a role for piRNAs in the silencing of repetitive elements in vertebrates. Furthermore, we show that piRNAs are Dicer independent and that their 3′ end likely carries a 2′O-Methyl modification.
