Author/Authors :
Min Wang، نويسنده , , Brian P. Ramos، نويسنده , , Constantinos D. Paspalas، نويسنده , , Yousheng Shu، نويسنده , , Arthur Simen، نويسنده , , Alvaro Duque، نويسنده , , Susheel Vijayraghavan، نويسنده , , Avis Brennan، نويسنده , , Anne Dudley، نويسنده , , Eric Nou، نويسنده , , James A. Mazer، نويسنده , , David A. McCormick، نويسنده , , Amy F.T. Arnsten، نويسنده ,
Abstract :
Spatial working memory (WM; i.e., “scratchpad” memory) is constantly updated to guide behavior based on representational knowledge of spatial position. It is maintained by spatially tuned, recurrent excitation within networks of prefrontal cortical (PFC) neurons, evident during delay periods in WM tasks. Stimulation of postsynaptic α2A adrenoceptors (α2A-ARs) is critical for WM. We report that α2A-AR stimulation strengthens WM through inhibition of cAMP, closing Hyperpolarization-activated Cyclic Nucleotide-gated (HCN) channels and strengthening the functional connectivity of PFC networks. Ultrastructurally, HCN channels and α2A-ARs were colocalized in dendritic spines in PFC. In electrophysiological studies, either α2A-AR stimulation, cAMP inhibition or HCN channel blockade enhanced spatially tuned delay-related firing of PFC neurons. Conversely, delay-related network firing collapsed under conditions of excessive cAMP. In behavioral studies, either blockade or knockdown of HCN1 channels in PFC improved WM performance. These data reveal a powerful mechanism for rapidly altering the strength of WM networks in PFC.
