Author/Authors :
Sylvain Feliciangeli، نويسنده , , Saïd Bendahhou، نويسنده , , Guillaume Sandoz، نويسنده , , Pierre Gounon ، نويسنده , , Markus Reichold، نويسنده , , Richard Warth، نويسنده , , Michel Lazdunski، نويسنده , , Jacques Barhanin، نويسنده , , Florian Lesage، نويسنده ,
Abstract :
A novel model for the regulation of cell excitability has recently been proposed. It originates from the observation that the background K+ channel K2P1 (TWIK1) may be silenced by sumoylation in Xenopus oocytes and that inactivation of the putative sumoylation site (mutation K274E) gives rise to robust current expression in transfected COS-7 cells. Here, we show that only the mutation K274E, and not K274R, is associated with an increase of K2P1 current density, suggesting a charge effect of K274E. Furthermore, we failed to observe any band shift by western blot analysis that would confirm an eventual sumoylation of K2P1 in COS-7 cells and oocytes.
