Long-term response of an integrated carbon paste based glucose biosensor

A biosensor containing integrated reference, auxiliary and two carbon paste based bioelectrodes shows linear calibration graphs for glucose concentrations > 16 mM. The biosensor response is performed on a renovated surface at an electrode potential of 60–80 mV; the capacity of the biosensor is 100 measurements. The long-term response of the biosensor relies on a glucose oxidase stability that depends on the structure of oil, water activity and stabilizer. The response of the biosensors containing silicone oil and stabilized glucose oxidase hardly changes during a period of three months at 35 °C and a relative humidity of 75%.