Catalytic effect of cetyltrimethylammonium bromide on the horseradish peroxidase-catalysed fluorogenic reaction between hydrogen peroxide and p-hydroxyphenylpropionic acid

A mechanistic study was made on the catalytic effect of cetyltrimethylammonium bromide (CTAB) on the horseradish peroxidase-catalysed fluorogenic reaction between para-substituted phenols and H2O2 with p-hydroxy-phenylpropionic acid as a model compound. The effect of CTAB was due to the interactions between the reaction products and the surfactant molecules. Novel behaviour of sodium chloride in the reaction was also observed.