Separation of polycyclic aromatic hydrocarbons by micellar electrokinetic chromatography with laser fluorescence detection

Polycyclic aromatic hydrocarbons (PAHs) were separated by micellar electrokinetic chromatography and were detected by laser fluorometry. The PAHs with large molecular weights were separated by using dimethylformamide as an organic modifier. A He-Cd laser (325 nm, 5 mW) or a blue semiconductor laser (415 nm, 50 μW) was used as an exciting light source. The detection limit (SN = 3) for perylene was 1.4 × 10−7 M under He-Cd laser excitation or 3.4 × 10−7 M under semiconductor laser excitation.