Hybrid tissue/enzyme biosensor for pectin

An amperometric biosensor for the determination of pectin was constructed by combining orange peel tissue as a pectinesterase source with a carbon paste alcohol electrode using alcohol oxidase. A slice of orange peel attached to the surface of the alcohol electrode deesterified pectin, the methanol generated by the reaction was monitored by the electrode. A linear response to pectin was observed in the concentration range of 0.1 to 0.9 g l−1. No interference from other carbohydrates was observed.