Determination of atrazine and alachlor in natural waters by a rapid-magnetic particle-based ELISA Influence of common cross-reactants: Deethylatrazine, deisopropylatrazine, simazine and metolachlor

The determination of atrazine and alachlor in various water types (distilled, ground and estuarine waters) in the presence of the common interfering compounds deethylatrazine, deisopropylatrazine and simazine (atrazine) and metolachlor (alachlor) has been investigated using direct rapid-magnetic particle-based ELISA. The interferences were studied at a fixed concentration of 0.5 μg l−1 atrazine or alachlor and at varying concentrations of the cross-reactants from 0.05 to 5 μg 1−1. Cross-reactivity values varied from 65–81% to 1.1% for deethylatrazine and metolachlor, respectively. Recommendations about the applications of the ELISA test to different types of water samples are given.