Spectrofluorimetric determination of berberine in oriental pharmaceutical preparations by flow-injection analysis coupled with liquid-liquid extraction

A sensitive and rapid determination of berberine was carried out by flow-injection analysis. Berberine was successfully extracted in the presence of sodium perchlorate. The calibration graph was linear over the range 4 × 10−9-1 × 10−6 mol dm−3. The sample throughput was 42 h−1.