Resolution of hidden minor peaks in multidetection chromatography by estimation of a single parameter in a 2-D transformation matrix

Unique resolution of multiwavelength chromatograms with a minor analyte completely overlapped by a major analyte in the time direction is accomplished by a new method. No selectivity in the wavelength region or any particular position of the minor analyte is assumed. The method estimates a single parameter in a two-dimensional transformation matrix which rotates loading vectors to pure spectra and scores vectors to pure chromatographie profiles. Selection of this single parameter is based on maintaining minimal variation of the derivative of the chromatographie profile of the major analyte in the two-analyte region.