Quantitative analysis of sulfadiazine using photochemically induced fluorescence detection in bulk solution and in a flow injection system

Room-temperature photochemically-induced fluorescence (RTPF) methods, using bulk solution and flow-injection analysis (FIA) were proposed for determining sulfadiazine (SDZ). The RTPF determination was based on the irradiation of SDZ with ultraviolet light. A methanol-water (50:50, vv) mixture was found to be the optimal solvent for the rapid, precise, and sensitive RTPF analysis of SDZ. Limits of detection were 40 ng/ml (bulk solution) and 90 ng/ml (FIA). The methods were applied for the determination of SDZ in pharmaceuticals. Satisfactory recoveries, ranging between 93 and 111.8%, were obtained.