Analytical characterisation and application of a p-benzoquinone mediated amperometric graphite sensor with covalently linked glucoseoxidase

The influence of the temperature, pH, p-benzoquinone and enzyme concentrations and speed of rotation of the electrode on the glucose determination with a p-benzoquinone mediated graphite sensor with covalently linked glucoseoxidase has been investigated. The results were applied to glucose analysis in concrete samples: blood serum, foodstuffs and pharmaceuticals. It was found that the proposed bioelectrocatalytic method for the determination of glucose is not inferior to the approved methods with respect to its accuracy; it is more rapid in comparison with the photometric method and it can be applied to determinations of low and high glucose concentrations.