Biosensors for analysis of ethanol in food: effect of the pasting liquid

An amperometric biosensor for ethanol was constructed by using an alcohol dehydrogenase and NAD+ modified carbon paste electrode. The sensor response to ethanol was studied using various pasting liquids. The electrode made with hexadecane as the pasting liquid showed an increase in sensitivity and linearity, compared to the one prepared with paraffin oil. This sensor was tested by assaying ethanol in beer and wine and the results compared favourably with spectrophotometric determinations.