Author/Authors :
Assadi، Najmeh Najmeh نويسنده Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran Assadi, Najmeh Najmeh , Zabihi ، Ebrahim نويسنده Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran Zabihi , Ebrahim , Khosravifarsani، Meysam Meysam نويسنده Cellular & Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran Khosravifarsani, Meysam Meysam , Khafri، Soraya نويسنده , , AkhavanNiaki، Haleh Haleh نويسنده Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran AkhavanNiaki, Haleh v , Amiri، Mehrangiz Mehrangiz نويسنده Cellular & Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran Amiri, Mehrangiz Mehrangiz , Shabestani Monfared، Ali Ali نويسنده Cellular & Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran Shabestani Monfared, Ali Ali
Abstract :
The adaptive response (AR) is a phenomenon by which cells exposure to sublethal doses of DNA-damaging agents (non-mutagenic dose of chemical or radiation), known as conditioning treatment (CT), leads to increased resistance to a subsequent exposure to a higher dose of the same or other agents, known as challenge treatment (CR). The adaptive response (AR) induced by radiation in human lymphocytes has been reported in a range of 1-20cGy pre-exposure. In this study, we investigated the adaptive response using 5cGy conditioning dose of gamma rays followed by 2 Gy challenging dose in peripheral human lymphocyte cells. Blood samples were taken from 30 female volunteers and this experiment was carried out by delivering 5 cGy gamma radiation followed by 2 Gy of challenging. Consequently, the number of micronuclei (MN) in binuclear lymphocyte cells was counted as an endpoint. The results showed that the mean frequency of micronuclei in binuclear lymphocytes which have received both conditioning and challenge doses are significantly reduced in comparison to those only exposed to 2 Gy (20.46±2.13, 30.2±3.29) (P < 0.01). The results showed the existence of an in vitro adaptive response in lymphocyte cell exposed to low dose of gamma radiations.
