Title of article :
Unbiased Mapping of Transcription Factor Binding Sites along Human Chromosomes 21 and 22 Points to Widespread Regulation of Noncoding RNAs
Author/Authors :
Patel، Sandeep K. نويسنده , , Bekiranov، Stefan نويسنده , , Gingeras، Thomas R. نويسنده , , Cawley، Simon نويسنده , , Ng، Huck H. نويسنده , , Kapranov، Philipp نويسنده , , Sekinger، Edward A. نويسنده , , Kampa، Dione نويسنده , , Piccolboni، Antonio نويسنده , , Sementchenko، Victor نويسنده , , Cheng، Jill نويسنده , , Williams، Alan J. نويسنده , , Wheeler، Raymond نويسنده , , Wong، Brant نويسنده , , Drenkow، Jorg نويسنده , , Yamanaka، Mark نويسنده , , Brubaker، Shane نويسنده , , Tammana، Hari نويسنده , , Helt، Gregg نويسنده , , Struhl، Kevin نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2004
Pages :
-498
From page :
499
To page :
0
Abstract :
Using high-density oligonucleotide arrays representing essentially all nonrepetitive sequences on human chromosomes 21 and 22, we map the binding sites in vivo for three DNA binding transcription factors, Sp1, cMyc, and p53, in an unbiased manner. This mapping reveals an unexpectedly large number of transcription factor binding site (TFBS) regions, with a minimal estimate of 12,000 for Sp1, 25,000 for cMyc, and 1600 for p53 when extrapolated to the full genome. Only 22% of these TFBS regions are located at the 5ʹ termini of protein-coding genes while 36% lie within or immediately 3ʹ to wellcharacterized genes and are significantly correlated with noncoding RNAs. A significant number of these noncoding RNAs are regulated in response to retinoic acid, and overlapping pairs of protein-coding and noncoding RNAs are often coregulated. Thus, the human genome contains roughly comparable numbers of protein-coding and noncoding genes that are bound by common transcription factors and regulated by common environmental signals.
Keywords :
NOx storage , Catalyst , NO oxidation , NOx storage/reduction catalysts , NOx release , Emissions
Journal title :
CELL
Serial Year :
2004
Journal title :
CELL
Record number :
102463
Link To Document :
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