Determination of bovine serum albumin by capillary zone electrophoresis with end-column amperometric detection at the carbon fiber microdisk array electrode

Capillary zone electrophoresis (CZE) was employed to the analysis of bovine serum albumin (BSA) using an end-column amperometric detector with a carbon fiber microdisk array electrode. The optimum conditions of separation and detection are 0.025 mol l−1 KH2PO4-0.02 mol l−1 NaOH for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, and 0.90 V vs. SCE for the detection potential. The limit of detection is 4 × 10−7 mol l−1 or 0.7 fmol (SN = 2). The method was applied to determining BSA in bovine serum.