Author/Authors :
Saber-Tehrani، Mohammad نويسنده , , Pourhabib، Akram نويسنده Department of Chemistry, Science and Research Branch, Islamic Azad University, P.O. Box: 1415-775, Tehran, Iran , , Husain، Seyed Waqif نويسنده Department of Chemistry, Science and Research Branch, Islamic Azad University, P.O. Box: 1415-775, Tehran, Iran , , Arvand، Majid نويسنده Department of Chemistry, Faculty of Science, University of Guilan, Namjoo Street, P.O. Box: 1914, Rasht, Iran ,
Abstract :
Quercetin belongs to the flavonol family. It is most abundant among the flavonoid
molecules. A method was developed for quantification of quercetin in apple and onion using
differential pulse voltammetry at graphene nanosheets modified glassy carbon electrode
(GNs/GCE) and UV spectrophotometry as reference method. Interest graphene centers on its
excellent mechanical, electrical, thermal and optical properties, it’s very high specific surface
area, and our ability to influence these properties through chemical functionalization. The
electrochemical behavior of quercetin at the GNs/GCE was investigated, and the results
indicated that the electrode reaction was controlled by adsorption. The anodic peak current
almost stops increasing after 260 s accumulation. This indicates that the accumulation of
quercetin at the electrode surface nearly reaches a saturation state after 260 s. The
accumulation potential was analyzed between -0.6 and 0.3 V, a maximum peak was achieved
at 0.2 V. Under the optimal conditions, the peak currents of DPV increased linearly with the
quercetin concentration in the range from 0.006 to 10 ?M and 10 to 100 ?M with limit of
detection 3.9 nM. The proposed method was successfully applied in the detection of
quercetin in foods.
