Title of article :
Covalent Immobilization of Phenylalanine Dehydrogenase on GlutaraldehydeModified Poly (3,4-ethylenedioxy) Thiophene: Poly(styrenesulfonate)/ Polyvinyl Alcohol Conducting Polymer Composite Films for El
Author/Authors :
Khanehzar، Ali نويسنده Enzyme Technology Lab., Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164, Tehran, Iran Khanehzar, Ali , Shadjou، Nasrin نويسنده Department of Analytical Chemistry, Faculty of Science, Payame Noor University(PNU), P.O. Box 58168- 45164, Khoy, Iran , , ShahbazMohamadi، Hamid نويسنده Enzyme Technology Lab., Genetic and Metabolism Group, Pasteur Institute of Iran, P.O. Box 13164, Tehran, Iran ShahbazMohamadi, Hamid , Hojati Emami، Shahriar نويسنده Biomedical Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran Hojati Emami, Shahriar , Hasanzadeh، Mohammad نويسنده Department of Analytical Chemistry, Faculty of Science, Payame Noor University(PNU), P.O. Box 58168- 45164, Khoy, Iran , , OMIDINIA، ESKANDAR نويسنده ,
Abstract :
A new strategy was developed to investigate conducting polymer poly(3,4-
ethylenedioxy)thiophene:poly(styrenesulfonate) (PEDOT:PSS) properties and potential for
enzymatic electrochemical detection of l-phenylalanine. PEDOT:PSS was blended with
polyvinyl alcohol (PVA) and further treated with glutaraldehyde (GA) to produce new
conducting polymer composite films of PEDOT:PSS/PVA-GA on indium(tin) oxide (ITO)-
coated glass slides. Phenylalanine dehydrogenase (PheDH) was covalently immobilized on
the glutaraldehyde modified conducting polymer composite films to produce
PEDOT:PSS/PVA-GA-PheDH films. Using this strategy, PEDOT:PSS remained chemically
unmodified and its electrical properties was maintained in a matrix containing glutaraldehyde
modified polyvinyl alcohol. Electrochemical behavior of the conducting polymer composite
films, before and after enzyme immobilization, was investigated using apotentiostat/galvanostat. Cyclic voltammograms showed an oxidation peak of about 0.63 V
for PEDOT:PSS/PVA-GA (PEDOT:PSS to PVA ratio=1/1) and about 0.73 V for
PEDOT:PSS/PVA-GA-PheDH in different concentrations of L-phenylalanine in
Glycine/KCl/KOH buffer solution (100 mM, pH=10.4). The redox properties of
PEDOT:PSS/PVA-GA films which is attributed to oxidation of PEDOT seems to contribute
to electron transfer to the electrode during enzymatic reaction. Results showed that the
system is suitable for determination of L-Phe in human urine for people with
phenylketonuria
