Flow injection chemiluminescence determination of pyrogallol Original Research Article

A sensitive and selective flow injection chemiluminescence method for the determination of pyrogallol over the range 5×10−7–3×10−5 M is described. The method is based on the chemiluminescence produced during the oxidation of pyrogallol by N-bromosuccinimide in alkaline media. The emission intensity is greatly enhanced if hydroxylammonium chloride and a cationic surfactant cetyltrimethylammonium bromide are also present in the reaction solution. Method development includes optimization of reagent concentrations and the flow conditions. The optimized flow injection procedure yielded a 2σ detection limit of 2×10−7 M pyrogallol, and a relative standard deviation of 1.6% (n=8) at 8×10−6 M pyrogallol.