Multi-enzymatic electrochemical sensor: field measurements and their optimisation Original Research Article

Using such enzymes as cholinesterases, urease and glucose oxidase, and electrolyte–insulator–semiconductor (EIS)-structures with silicon nitride ion-sensitive layers as transducers investigations have been performed on the determination of heavy metal ions and phosphororganic pesticides in solutions. The content of toxic elements indicated above was determined by estimation of the residual activity of enzymatic membranes after the injection of analysed samples. The optimal conditions were chosen for analysis of heavy metal ions and phosphororganic pesticides in potato and cabbage saps. It was shown that multienzyme analysis followed by mathematical processing is one of the effective approaches to develop computer-controlled sensor arrays for toxic substrates.