Development of an electrochemical flow injection immunoassay (FIIA) for the real-time monitoring of biospecific interactions Original Research Article

Not only are sensors a revolution in analysis; they themselves are also experiencing a revolution brought about by parallel developments in sensor fabrication techniques and materials, polymer chemistry, signal processing methodologies, the increased use of biomolecular processes as a means of analyte detection, and the coupling of sensors to other techniques such as flow injection analysis. Many of these developments have been incorporated into the present study, which we are undertaking in the development of our immunosensor technology. The system described here utilises screen-printed electrodes which are low-cost, disposable devices that are simple to fabricate. Incorporated into our sensor is the electroactive polymer, polyaniline, which brings about mediatorless redox coupling between the electrode and biomolecular components attached to the polymer surface. This system also utilises enzyme-labelled antibodies as the biomolecular recognition component for the analysis of the test analyte, biotin. The system has also been integrated into a flow injection system. This has led to the monitoring of real-time antibody-antigen interactions using electrochemical methods and foreshadows the development of single-step immunosensors.