Bioanalytics: detailed insight into bioprocesses Original Research Article

The principles of bioanalytical systems for an on-line bioprocess monitoring are described within this paper. These sensor systems can be interfaced to the bioprocess in different ways according to the needs of the single bioprocess. Modular systems are necessary, which can fit exactly to the needs of the single process. Invasive as well as non-invasive bioanalytical tools are described and discussed in detail. Immunosensors give the possibility to monitor high molecular weight components within short time intervals. Non-invasive optical sensors allow the direct monitoring of various analytes such as oxygen pH for the complex fluorescence behavior of the bioprocess medium. These so-called fluorescence sensors offer the possibility to monitor intra- as well as extracellular components without interfering with the bioprocess. An industrial example for the application of bioanalytical tools for a process optimization are presented in this application. Here a biosensor system is used to optimize the downstreaming of molasses on a technical scale. The economic as well ecological advantages are discussed.