Liquid chromatography — electrochemical detector for the determination of glucose in rat brain combined with in vivo microdialysis Original Research Article

In this paper, microdialysis sampling was combined with liquid chromatography with electrochemical detection (LC-ECD) and glucose in rat brain was determined. A cobalt tetraaminophthalocyanine (CoTAPC)/glucose oxidase (GOD)/Nafion film-coated glassy carbon electrode was used to selectively detect the hydrogen peroxide generated enzymatically in the enzyme reactors, without any interference from electroactive species such as l-ascorbate, urate, proteins. The signal current was linearly related to the glucose concentration in the range of 1.0×10−6–5.0×10−3 mol/l at +0.5 V (versus Ag/AgCl) with a detection limit of 5.0×10−7 mol/l. To measure glucose in rat brain, microdialysis sampling was employed. The dialysate from the microdialysis system was injected into system. The result was 0.74±0.10 mmol/l (n=4).