Coupling conducting polymers and mediated electrochemical responses for the detection of Listeria Original Research Article

Different signal generation techniques were investigated for the development of a biosensor for Listeria monocytogenes. Conventional amperometry at an antibody-containing polypyrrole film electrode was found to be unsuccessful in detecting levels below 106 cells ml−1. More successful was the coupling of a covalently modified film with the use of electron mediators in a single device. This sensor was capable of reproducibly detecting Listeria at levels of 105 cells ml−1 in 30 min.