Application of liquid chromatography–mass spectrometry in the determination of oxazaphosphorines and their metabolites Review Article

This article presents an overview of the methods of bioanalysis of oxazaphosphorines and their metabolites, in particular liquid chromatography–mass spectrometry (LC–MS) and liquid chromatography–tandem mass spectrometry (LC–MS–MS) assays. Especially for the molecules that do not contain chromophores as oxazaphosphorines the mass spectrometry took an important place in chromatographic analysis. For trace analysis the LC–MS–MS methods are particularly well suited. Above all gas chromatography–mass spectrometry (GC–MS) assays were applied for the determination of these substances, in addition, LC–MS methods and other high-performance liquid chromatography (HPLC) or GC methods were included in the consideration.