Cytotoxicity testing of model compounds through cell adhesion to a pattern of collagen matrix Original Research Article

A pattern of fluorescein isothiocyanate labeled collagen (FITC-collagen) can be fabricated on a substrate by photolithography, which only to human venous endothelial cells specifically adhere. The cytotoxicity of six organic compounds is tested through this adhesion process, by counting the FITC-collagen film islands that have endothelial cells in a binary system, as well as by counting all of the cells anchored to the film islands. The film pattern, 50 μm in diameter with 100 μm spaces, also plays a crucial role to enable chemical cytotoxicity testing with 1 h incubation.