Chemiluminescent determination of Ce(IV) using Cypridina luciferin analog Original Research Article

A cerium(IV)-induced Cypridina luciferin analog (CLA) chemiluminescence is reported. This Ce(IV) triggered luciferin analog CL is enhanced about 5-fold by 0.5% (v/v) Tween 20. Using this enhanced luciferin analog-dependent chemiluminescence, a lower limit detection of 25 nM was determined for Ce(IV). Ce(III) could not generate this luciferin analog-dependent chemiluminescence. Oxidising agents such as potassium permanganate and potassium dichromate were also without effect. While the tested scavengers of reactive species such as mannitol, superoxide dismutase and catalase had no specific effect on the luminescence, this Ce(IV)-elicited luciferin analog chemiluminsecent reaction was inhibited when the reaction was performed under anaerobic condition, indicating that molecular oxygen participates in the reaction mechanism leading to the formation of the light-emitting product. A mechanism is proposed for this chemiluminescent reaction which is consistent with the presented data.