Flow-injection chemiluminescence sensor for the determination of isoniazid Original Research Article

By electrostatically immobilizing the chemiluminescence (CL) reagent luminol and periodate on anion exchange resin separately, a novel CL sensor for isoniazid combined with flow-injection analysis was developed. Isoniazid was sensed by its enhancing effect on the weak CL reaction between luminol and periodate, which were eluted from the ion exchange column. The calibration graph is linear in the range 8.0×10−9 to 1.0×10−6 mol l−1 and the detection limit is 4.2×10−9mol l−1 isoniazid (3σ). A complete analysis could be performed in 1 min with a relative standard deviation 2.0% (n=9). The sensor could be reused for over 400 times and has been applied successfully to the determination of isoniazid in pharmaceutical preparations.