Determination of six components in Rhubarb by cyclodextrin-modified micellar electrokinetic chromatography using a mixed micellar system of sodium cholate and sodium taurocholate Original Research Article

A cyclodextrin-modified micellar electrokinetic chromatographic method was established to determine five anthraquinone derivatives and a distyrene derivative in Rhubarb. The six components were successfully separated by using the mixed micellar system consisting of 20 mmol/l SC and 20 mmol/l STC with 15 mmol/l β-CD in the BGE of 20 mmol/l borax buffer (pH 11) in less than 20 min. Effect of SC and STC concentrations, pH of BGE and concentration of β-CD on the separation was studied. The analytical performance of the method was discussed in terms of linearity response, precision, detection limits, quantitation limits and recoveries. The method developed was applied to the determination of three commercial Rhubarb samples. The results obtained agreed with those of the reported documents.