Influence of selectivity and sensitivity parameters on detection limits in multivariate curve resolution of chromatographic second-order data Original Research Article

We have established a procedure for calculating limits of detection for second-order data. One of the steps involves curve resolution by iterative target transformation factor analysis (ITTFA) and we have checked some experimental factors that affect the efficiency of resolution by ITTFA. Therefore, they directly affect the estimation of the limits of detection (LOD). In this paper, we describe the quality of the LOD estimator as a function of the performance characteristics of a determination with high performance liquid chromatography (HPLC)-diode array detection (DAD) (sensitivity and selectivity of spectra and chromatograms) and advise the end user about how he can improve it by modifying these experimental variables.