Proteomics in cancer research: Methods and application of array-based protein profiling technologies Review Article

With the human genome sequence now determined, the field of molecular medicine is moving beyond genomics to proteomics, the large-scale analysis of proteins. It is now possible to examine the expression of more than 1000 proteins using mass spectrometry technology coupled with various separation methods. Microarray technology is a new and efficient approach, for extracting relevant biomedical data and has a wide range of applications. It provides a versatile tool to study protein–protein, protein–nucleic acid, protein–lipid, enzyme–substrate and protein–drug interactions. This review paper will explore the key themes in proteomics and their application in clinical cancer research.