Title of article :
Integrated analysis of serum and liver metabonome in liver transplanted rats by gas chromatography coupled with mass spectrometry Original Research Article
Author/Authors :
Yi Wang، نويسنده , , Yi Tao، نويسنده , , Yunjin Lin، نويسنده , , Liang Liang، نويسنده , , Yi Wu، نويسنده , , Haibin Qu، نويسنده , , Tingbo Liang، نويسنده , , Yiyu Cheng، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Abstract :
In this paper, we present a metabonomic method for the investigation of abnormal metabolic process in both serum and liver tissue of liver transplanted rats. Syngeneic transplantation was performed on male Lewis rats. The serum and grafted liver on day 1, 3, and 7 post-transplant were collected to analyze endogenous metabolites using gas chromatography coupled with mass spectrometry (GC–MS). The method was validated with acceptable linearity, precision, and repeatability. Thirty-four metabolites in serum and 29 metabolites in liver were identified. Results of correlation analysis illustrated metabolites with similar function exhibited similar variations in liver and serum. The data processed by principle component analysis (PCA) showed time-dependent biochemical variations. As a consequence, the present study may offer specific putative pathways in the pathophysiological mechanism of orthotopic liver transplantation.
Keywords :
Metabolomic profile , Integrated analysis , Gas chromatography–mass spectrometry , Liver transplantation
Journal title :
Analytica Chimica Acta
Journal title :
Analytica Chimica Acta