Author/Authors :
Sheibani، Ali نويسنده Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran , , Shishehbore، M. Reza نويسنده Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran , , Vafaie-Shahi، Shohrah نويسنده Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran ,
Abstract :
In this study, a rapid, simple and sensitive ion mobility spectrometry (IMS) method with corona discharge as ionization source was described for the morphine determination in human urine. Morphine was extracted and purified from urine samples using solid phase extraction procedure with C18 column. It can offer the clean extracts which no extra peaks were observed in IMS. Under operating experimental conditions (Temperature; injection: 220 and oven: 180 oC, Flow rate; carrier: 300 and drift: 600 mL min-1, Voltage; corona: 2200 and drift: 6700 V), developed method showed good linearity in the ranges of 0.44 to 6.91 and 6.91 to 22 ng mL-1 with correlation coefficients (R2) of 0.9979 and 0.9966, respectively. The limit of detection was 0.1 ng mL-1, and precision as relative standard deviation was 11%. The capability of the proposed method was evaluated by the analysis of human urine as a real sample that satisfactory results were obtained.
