Revised Stereochemistry and Biosynthesis of Seragakinone A

The relative stereochemistry of seragakinone A (1), an anthracycline-derived pentacyclic fungal metabolite, was revised by X-ray analysis. The tetracyclic skeleton (C-1–C-14) and the C5 moiety (C-1′–C-5′) of 1 were shown to be biosynthesized via decaketide and mevalonate pathway, respectively, on the basis of incorporation experiments.