Title of article
Regioselective biocatalytic hydrolysis of (E,Z)-2-methyl-2-butenenitrile for production of (E)-2-methyl-2-butenoic acid
Author/Authors
Eugenia C Hann، نويسنده , , Amy E Sigmund، نويسنده , , Susan K Fager، نويسنده , , Frederick B Cooling، نويسنده , , John E Gavagan، نويسنده , , Michael G Bramucci، نويسنده , , Sarita Chauhan، نويسنده , , Mark S Payne، نويسنده , , Robert DiCosimo، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2004
Pages
5
From page
577
To page
581
Abstract
Acidovorax facilis 72W nitrilase catalyzed the regioselective hydrolysis of (E,Z)-2-methyl-2-butenenitrile, producing only (E)-2-methyl-2-butenoic acid with no detectable conversion of (Z)-2-methyl-2-butenenitrile. (E)-2-Methyl-2-butenoic acid, produced in aqueous solution as the ammonium salt, was readily separated from (Z)-2-methyl-2-butenenitrile, and isolated in high yield and purity. The combination of nitrile hydratase and amidase activities of several Comamonas testosteroni strains were also highly regioselective for the production of (E)-2-methyl-2-butenoic acid from (E,Z)-2-methyl-2-butenenitrile.
Keywords
Nitrile hydratase , Amidase , Nitrilase , Enzyme catalysis , Angelic acid , Tiglic acid
Journal title
Tetrahedron
Serial Year
2004
Journal title
Tetrahedron
Record number
1084692
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