Author/Authors :
Thomas Purkarthofer، نويسنده , , Wolfgang Skranc، نويسنده , , Hansj?rg Weber، نويسنده , , Herfried Griengl، نويسنده , , Marcel Wubbolts، نويسنده , , Gerald Scholz، نويسنده , , Peter P?chlauer، نويسنده ,
Abstract :
In a chemoenzymatic one-pot reaction of ethyl cyanoformate with benzaldehyde catalyzed by the hydroxynitrile lyase from Prunus amygdalus ethoxycarbonylated (R)-mandelonitrile is formed in a highly enatioselective manner. The reaction was performed both in aqueous and organic media. 1H NMR investigations revealed a two-step procedure consisting of an enzyme-catalyzed addition of HCN, generated by hydrolysis of ethyl cyanoformate, to the aldehyde followed by ethoxycarbonylation of the free cyanohydrin in a second step
Keywords :
Cyanohydrins , hydroxynitrile lyase (PaHNL) , Chemoenzymatic synthesis , ethyl cyanoformate
