New fluorescent probes for testing combinatorial catalysts with phosphodiesterase and esterase activities

Combinatorial development of new catalysts with phosphodiesterase and esterase activities requires specific fluorescent probes for rapid visual detection of hydrolytic activity. Such fluorescent probes have been synthesized with special attention to solubility in water and stability towards spontaneous hydrolysis at a given pH. The probes reported here include compound based on a fluorescein fluorophore, compound for FRET-detection of phosphodiester hydrolysis and compound based on a quinolinium fluorophore.