Synthesis of oligosaccharides as potential novel food components and upscaled enzymatic reaction employing the β-galactosidase from bovine testes

The β-galactosidase from bovine testes (EC 3.2.1.23) promotes the transfer of a galactose unit to glucose or galactose-containing residues in manifold derivatives, establishing β1→3 linkages. The synthesis of several potentially biologically important oligosaccharides β-d-Galp-(1→3)-α-d-Glcp-(1→2)-β-d-Fruf , β-d-Galp-(1→3)-β-d-Galp-(1→4)-α,β-d-Glcp , β-d-Galp-(1→3)-α-d-Glcp-(1→4)-d-Glcp-ol/Manp-ol , β-d-Galp-(1→3)-α-d-Glcp-(1→6)-β-d-Fruf , β-d-Galp-(1→3)-α-d-Glcp-(1→6)-[α-d-Glcp-(1↔2)]-β-d-Fruf , α-d-Galp-(1→6)-[β-d-Galp-(1→3)]-α-d-Glcp-(1↔2)-β-d-Fruf , β-d-Galp-(1→3)-α-d-Glcp-(1↔2)-β-d-Fruf-(1↔2)-β-d-Fruf has been reached in yields between 7 and 44% by implementation of this specific enzyme. In addition, we found that it is feasible to gain high yields without an enzyme-specific buffer and even making upscaled preparation on a gram scale.