Author/Authors :
Zarei ، Leila نويسنده Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Sadrkhanlou، Rajabali نويسنده Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Shahrooz، Rasoul نويسنده Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , , Malekinejad، Hassan نويسنده Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran , , Eilkhanizadeh، Behroz نويسنده Department of Pathology, Faculty of Medicine, University of medical Sciences, Urmia, Iran , , Ahmadi، Abbas نويسنده Department of Embryology, Faculty of Veterinary, University of Urmia, Urmia, Iran ,
Abstract :
This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE) and vitamin E (Vit E) on sperm quality parameters in the methotrexate (MTX)-treated mice. Forty-eight young adult male mice (8-12 weeks) were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (20 mg kg-1, ip, once weekly), MTX + CMFE (250 mg kg-1), MTX + CMFE (500 mg kg-1), MTX + CMFE (1000 mg kg-1), and MTX + Vit E (100 IU kg-1, po) for 35 consecutive days. On day 35, after euthanasia the epididymal sperms were isolated. Then the total mean sperm count, sperm viability and motility were determined. The total antioxidant capacity (TAOC) of all experimental groups were also evaluated. The MTX-treated animals showed a significant changes in all parameters of sperm quality assessment compared to the control group. Both Vit E and CMFE were able to protect from MTX-induced effects on sperm maturity and DNA damage. Co-administration of MTX and CMFE and/or Vit E resulted in protection from MTX-reduced TAOC. In conclusion, these data suggested that MTX administration could adversely affect the sperm quality. Moreover, the protective effect of Vit E and CMFE on MTX-induced sperm toxicity was also documented.
