Lipoxygenase-1 Mass-Transfer Coefficient in Aqueous Two-Phase System Using Spray Extraction Column

Extraction of lipoxygenase-1 was investigated by using the aqueous twophase system formed by sodium sulfate-poly(ethylene glycol)-buffer. The extraction was performed in a 47-mm inner diameter spray column, operating in a semibatch manner. The effects of dispersed-phase flow rate and phase compositions on fractional dispersed-phase hold up ((epsilon)D) and volumetric mass-transfer coefficient (KDa) were respectively studied. It was found that the hold-up and volumetric mass-transfer coefficient increased with an increase in dispersed-phase velocity and decreased with increasing phase composition. Correlations have been developed by nonlinear regression for estimation of fractional dispersed-phase hold up and volumetric mass-transfer coefficients.