Solid-phase synthesis of hydrazinopeptides in Boc and Fmoc strategies monitored by HR-MAS NMR

With the aim of finding good conditions to incorporate hydrazino moieties into peptidic sequences of biological interest, we developed two strategies towards solid-phase synthesis of hydrazinopeptides: a stepwise methodology in which a hydrazinoacid was introduced as a normal aminoacid and a semi-convergent one in which the hydrazino moiety is incorporated as a pseudodipeptide building block. HR-MAS NMR analyses were run in order to monitor the reaction and to improve the reaction conditions.