Author/Authors :
Yulin Ren، نويسنده , , Daniel D. Lantvit، نويسنده , , Esperanza J. Carcache de-Blanco، نويسنده , , Leonardus B.S. Kardono، نويسنده , , Soedarsono Riswan، نويسنده , , Heebyung Chai، نويسنده , , Charles E. Cottrell، نويسنده , , Norman R. Farnsworth، نويسنده , , Steven M. Swanson، نويسنده , , Yuanqing Ding، نويسنده , , Xing-Cong Li، نويسنده , , Jannie P.J. Marais، نويسنده , , Daneel Ferreira، نويسنده , , A. Dou، نويسنده ,
Abstract :
A new biflavonoid (1), a new xanthone enantiomer (2), five new caged xanthones (3–7), and several known compounds were isolated from the stem bark of Garcinia lateriflora, collected in Indonesia. The structures of the new compounds were determined by analysis of spectroscopic data, and the absolute configuration of the caged xanthones was shown for the first time at carbons 5, 7, 8, 8a, 10a, and 27, by analysis of COSY and NOESY NMR and ECD spectra. The biflavonoids exhibited proteasome-inhibitory activity, and the known compound, morelloflavone (8) was found to have the greatest potency (IC50=1.3 μM). The caged xanthones were cytotoxic toward HT-29 cells, with the known compound, morellic acid (10) being the most active (ED50=0.36 μM). However, when tested in an in vivo hollow fiber assay, it was inactive at the highest dose tested (20 mg/kg).
Keywords :
Garcinia lateriflora , biflavonoids , Caged xanthones , Proteasome inhibition , Cytotoxicity
