15N-D-labeled ionic probes for mass spectrometry

An effective 15N- and deuterium (D)-labeled 2,6-bis(oxazolin-2-yl)pyridine (pybox)–La complex based probe ionization method that produces a distinct isotopic shift was developed. The distinct isotopic shift was detected by using the newly synthesized 15N-D-labeled pybox complexes. Moreover, O-[3-(tetramethylpybox)-propyl]-hydroxylamine (oxime–TMpybox) was prepared for attachment to the carbonyl group of the target molecule. Distinct isotopic shifts and multiple charged ions were detected for various compounds having amino, thiol, carboxyl, and carbonyl groups and fullerenes, using the TMpybox ionic probe series in cold-spray ionization mass spectrometry.