Abstract :
The current work presents analytical procedures for simultaneous determination of tarabine PFS and adriblastina by micellar electrokinetic chromatography (MEKC) and liquid chromatography (LC). For MEKC analysis, separations and identifications were accomplished using uncoated fused-silica capillary with hydrodynamic injections in the presence of 50 mM borate/phophate pH 8.7 and 100 mM SDS. The migration times of tarabine PFS and adriblastina were found to be 2.70 and 6.40 min, respectively. Calibration curves were established for 10-300 ng/mL (r = 0.998) tarabine PFS and for 8120 (mu)g/mL (r = 0.999) adriblastina. For LC analysis, separations were performed on teicoplanin stationary phase with reversed mobile phase containing methanol:buffer pH 4.05 (20:80%, v/v) at 285 nm. The retention times of tarabine PFS and adriblastina were 5.18 and 7.20 min, respectively. Calibration curves were established for 3-90 (mu)g/mL (r = 0.998) tarabine PFS and for 10-120 (mu)g/mL (r = 0.999) adriblastina. Both MEKC and LC methods were applied for the simultaneous determination of analytes in urine samples.
